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Satisfactory production, with a maximum lipid productivity of 2.3 g/L, lipid content of 39.8% (w/w), and cell dry weight (CDW) of 5.7 g/L, was obtained at a culture time of 48 h.
The difference of the carbon source consumption for maximum lipid productivity between simulations with and without citrate production was determined and used as a basis for the calculation of the feed strategy for fed batch cultivation.
The optimized conditions for maximum lipid productivity were found to be 5.96 g L−1 glucose, 4.12 g L−1 acetate, 0.73 g L−1 nitrate and incubation period of 10 days.
The maximum CDW was obtained at pH 7.0, while the maximum lipid productivity and content were obtained at pH 6.5.
The maximum lipid productivity (223.2 mg L-1d-1) and lipid content (59.4%) were obtained when the salinity gradient was operated at an increment of 0.5% per day.
Satisfactory productivities were obtained after 48 h of batch fermentation in a bioreactor, with a maximum lipid productivity of 2.3 g/L, lipid content of 39.8% (w/w), and cell dry weight (CDW) of 5.7 g/L.
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It was found that the maximum lipid productivities of 63.52 mg L−1 d−1 occurred after 24 h N-shift.
Under the fed-batch culture condition, the maximum biomass and lipid productivity were 24.01 g/L and 1.19 g/L/d, respectively.
The maximum lipid yield and productivity were achieved at C/N 80 for Rhodosporidium TJUWZ4 whereas for Cryptococcus TJUWZA11 it was 120 N (Table 3).
Feng et al. (2011) reported the same results, C. pyrenoidosa had the maximum lipid content and productivity under the N replete medium rather than N deplete medium.
While biomass productivity was 12% higher in the bubble column bioreactor (68.2 gdw m−2 day−1), maximum lipid volumetric production (0.19 gLipid L−1) was found in a gas-lift bioreactor sparged with 6% carbon dioxide due to hydrodynamic and light stresses.
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