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After photobleaching, Raman hyperspectral imaging was performed to locate an area with the maximum lipid contribution to Raman spectra, as described in " Methods" (see also Additional file 1).
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The maximum lipid yield, especially for oxygenated compounds was in the window of 150 250 °C.
Indeed, N-starvation does not influence lipid production, however, maximum lipid content (4.3 ± 0.17 g/L), lipid productivity (0.79 ± 0.01 g/L day) and lipid yield (175.56 ± 21.9 mg/g glucose) was obtained with MN supplementation (Table 2).
Maximum lipid content was obtained at 120 h of cultivation in all treatments.
The maximum lipid production was obtained at 12% w/w.
For this study, we defined the optimal time of MTBE-based lipid extraction at which maximum lipid recovery is achieved.
The maximum lipid concentration (13.77 g/L) with lipid content of 33.1% w/w was observed on day 10.
The maximum CDW was obtained at pH 7.0, while the maximum lipid productivity and content were obtained at pH 6.5.
The maximum lipid content appeared to be dependent on the availability of nitrogen.
Low density conditions were applied for all strains to achieve maximum lipid accumulation levels (Table 1).
The maximum lipid accumulation capacity of the culture was 54% on VSS mass basis.
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