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The scale-up under different fermentation conditions based on S2 medium led to a maximum lipid content of 65%.
The model estimated that a maximum lipid content of 28.1% wt/wt in dry biomass, a lipid yield of 1.2 g/L and a cell mass yield of 4 g/L could be obtained when a mixture of expired soft drinks and olive mill wastewater (25% in water, v/v) is used as substrate at percentages 62.4% and 37.6%, respectively.
Maximum lipid content was obtained at 120 h of cultivation in all treatments.
Feng et al. (2011) reported the same results, C. pyrenoidosa had the maximum lipid content and productivity under the N replete medium rather than N deplete medium.
Indeed, N-starvation does not influence lipid production, however, maximum lipid content (4.3 ± 0.17 g/L), lipid productivity (0.79 ± 0.01 g/L day) and lipid yield (175.56 ± 21.9 mg/g glucose) was obtained with MN supplementation (Table 2).
The maximum lipid content was observed in the photoautotrophic group, in agreement with the results reported by Liang et al. (2009) and Heredia-Arroyo et al. (2011) who also focused on C. vulgaris.
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The maximum total lipid content of 44.8% occurred when peptone was utilized as the nitrogen source, but the biomass concentration and specific growth rate were only 1.16 g L-1 and 0.552 d-1, respectively.
The maximum CDW was obtained at pH 7.0, while the maximum lipid productivity and content were obtained at pH 6.5.
The maximum lipid and triglyceride contents (56.1and 15.1% of dry weight respectively) were gained at 25% nitrogen concentration followed by 50% nitrogen concentration (45.2 and 12.5 of dry weight respectively), while the minimum contents (36.1 and 10.7% of dry weight respectively) were obtained at 75% nitrogen concentration.
The optimal conditions for attaining both maximum biomass and total lipid content were selected to be 28.26 °C, 76.64 μmol m−2 s−1 and 4.00% CO2.
The maximum lipid concentration (13.77 g/L) with lipid content of 33.1% w/w was observed on day 10.
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