Sentence examples for maximum lipase production from inspiring English sources
The maximum lipase production (9.40 U mL−1) was obtained under optimal condition.
KH2PO4 was found to be responsible for maximum lipase production of 5.59 IU/ml, experimental and 5.03 IU/ml, predicted.
The optimum values of investigated variables for the maximum lipase production were 6.0% Mahua cake, 2.0% glucose, 0.2% MnCl2 and 0.2%KH2PO44.
The validity of the model developed was verified, and the optimum medium containing 0.45% (w/v) peptone, 0.65% (v/v) Tween-80 and 2.2% (v/v) inoculum led to a maximum lipase production of 20.26 U/ml, which was 5.19-fold higher than the unoptimized medium.
The central composite design was employed, and the optimal medium constituents for maximum lipase production (1355.81 U/ml) were determined to be tuna powder (14.58 g/l), olive oil (5.05 ml/l); NaCl (72.42 g/l), temperature (45 °C) and pH 9.0.
RSM predicted that incubation time, inoculum density and oil were required at their higher levels (36 h, 3% (v/v) and 3% (v/v), respectively) while glucose and yeast extract were required at their minimal levels for maximum lipase production in shake flasks.
At pH 6 and 9, maximal lipase production was observed.
After derivation of the quadratic equation and calculation (according to Design-Expert 8.0), the concentrations of three factors in the optimum medium for maximum lipase activity production were predicted as 10.5 g/L corn starch, 35.4 g/L soybean meal, and 10.9 g/L soybean oil.
All cultures were developed in triplicate and the results were assayed for biomass, maximum lipase activity, biomass yield on substrate, lipase yield on substrate, biomass productivity, and specific rate of lipase production.
As expected, untreated coconut dregs did not favour lipase production when only a maximum of 44 U/mL lipase was obtained.
Response Surface Methodology (RS M based on central composite design was used to optimize the culture conditions for lipase production, concluding that the maximum levels of lipase biosynthesis were obtained at 21.6 °C and pH 6.9.
