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Both procedures (Neighbor-Joining and Maximum Likelihood) use the bootstrap method with 100 replicates for testing the phylogeny.
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Parameter estimation is done by maximum likelihood using first-passage time probabilities of diffusion processes [25].
The three models have been estimated by maximum likelihood using the equations given in Section 3.4.
The phylogenetic tree was created by the method of maximum likelihood using the PhyML (HYK85 model) with 100 bootstrap replicates.
The phylogenetic analysis was conducted by the method of maximum likelihood using PhyML with 100 bootstrap replicates.
The estimation method was maximum likelihood using the covariance matrix according to the suggestions of Hair et al. (2010).
Odds ratios (ORs) were estimated by maximum likelihood using unconditional logistic regression.
The random-effects Poisson model was fitted via maximum likelihood using the xtpoisson command.
Ancestral sequences were reconstructed by maximum likelihood using the software PAML v4 [23].
The combined nucleotide sequences were also analyzed under maximum likelihood using Bayesian inference with the program MrBayes v3.1.2 [205], [205].
All alignments were subject to distance phylogenetic reconstruction in MEGA4 [63], maximum likelihood using PhyML3 [67] and PAUP*4b10 (D.L. Swofford, 2001, PAUP*, Sunderland, MA: Sinauer Associates), and Bayesian using MrBayes3.1.2 [68].
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