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Figure 1 Best-scoring maximum likelihood phylogram.
Figure 1 Simplified phylogenetic tree based on the best-scoring maximum likelihood phylogram of Chung et al. ([2014]).
A maximum likelihood phylogram was constructed using a selection of α1 and HMG core domains from representative taxa (Figure 3).
MEGA5.2 [ 39] was used to produce a maximum likelihood phylogram.
DOI: http://dx.doi.org/10.7554/eLife.05503.003 10.7554/eLiFigure03.004 Figure 1 figure supplement 1. Maximum likelihood phylogram resulting from analysis of untrimmed 516-gene matrix.
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In order to summarize the results, the bootstrap values and genetic distances, estimated using the uncorrected 'p' distance setting, were added to the final maximum likelihood phylograms.
When the maximum likelihood phylograms from each subject's sequences were assessed for compartmentalization between genital and blood viruses the outcome varied across the four assays.
To further assess the likelihood that HIV-1 DNA genital sequences originated in genital tract, we evaluated the tissue origin of the nearest neighbor to each cell-associated genital sequence in the maximum likelihood phylograms of all 13 subjects.
In addition, maximum-likelihood phylograms and non-parametric bootstrap values (100 replicates) were generated with the program Garli (Version 0.951) using default search options under the GTR + gamma + I model for each of the three newly reported gene alignments with parameters estimated from the data.
In all analyses, we used the best scoring maximum likelihood (ML) phylogram of Massoni et al. [ 33] obtained with the 12-marker RAxML analysis excluding Hydnora.
The midpoint rooted maximum likelihood (ML) phylogram placed the potential mammalian NIRVs within the Mononegavirales, and revealed that the mammalian sequences are more closely related to filoviruses than to Paramyxoviruses (Figs. 2, 3).
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