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We also constructed a maximum likelihood gene tree with PHYLIP's dnaml [84] and obtained the same topology.
From theses alignments we inferred the maximum likelihood gene trees, which were subsequently used to reconstruct the species' phylogeny.
We then estimated a maximum likelihood gene tree for each locus using PHYML [ 48] using the appropriate model selected by MrAIC.
Hence, we aligned the homologs resulting from the BLAST search for the 579 Blochmannia genes and obtained the corresponding maximum likelihood gene trees.
A distance matrix (Additional file 1) was generated for each maximum likelihood gene tree, showing the relative branch lengths between each organism for each particular protein.
The resultant Bayesian gene tree is shown in Figure 2 with the support values from both the Bayesian gene tree and the maximum likelihood gene tree shown on the tree in Figure 2i.
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We then constructed neighbor-joining (NJ) trees [ 59] based on a maximum-likelihood gene content distance measurement [ 60].
Using a maximum-likelihood gene level analysis we obtained evidence for positive selection on the ANG gene.
After T-Coffee alignment, we estimated maximum-likelihood gene trees for the alignments with PhyML (Guindon and Gascuel 2003).
We evaluated model fit by comparing nDC for the 'best' cyt b maximum-likelihood gene tree to that from the simulated gene trees (treated as rooted).
We generated maximum-likelihood gene trees for all loci (NGS and Sanger-sequenced loci) using RAxML v.7.0.4 [ 50] under the GTRGAMMA model.
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