A nano-bubble layer accumulates oxygen in a very narrow range occurring between the damage maximum, DP, and the mean projected ion range, RP.
The AGC was set for high dynamic range with a maximum ion injection time of 100 ms. Electrospray emitters were fabricated by chemically etching fused-silica capillary tubing with a 10 μm i.d. and 150 μm o.d.
The AGC was set for high dynamic range (3× 10) with a maximum ion injection time of 100 ms. The front end ion optics on both instruments were modified to include a SPIN-MS interface.
Due to the finite ion range during implantation, however, the maximum thickness of such coatings is limited.
For the analysis of unlabeled peptides, the instrument method consisted of a full MS scan (scan range 375 1550 m/z, with 30K FWHM resolution @ m/z = 400, target value 2×106, maximum ion injection time of 500 ms) followed by data-dependent CID scans of the six most intense precursor ions.
The source magnet is adjusted to maximum electron trap current rather than for maximum ion current.
Collision energy was set to 45, maximum inject time was 250 ms and maximum ion count was 1 × 10 counts.
Maximum ion injection time was 100 ms, and normalized collision energy was 35%.
Maximum ion injection times for MS1 and MS2 were 500 and 100 ms, respectively.
We applied the low-resolution mode (m/Δm = 300) to obtain maximum ion transmission.
