Maximum ion injection time was 100 ms, and normalized collision energy was 35%.
Maximum ion injection times for MS1 and MS2 were 500 and 100 ms, respectively.
Collision energy was set to 45, maximum inject time was 250 ms and maximum ion count was 1 × 10 counts.
The source magnet is adjusted to maximum electron trap current rather than for maximum ion current.
We applied the low-resolution mode (m/Δm = 300) to obtain maximum ion transmission.
The maximum ion time was 200 ms for MS and MSn.
The maximum ion count accumulated in the trap was set as 30000.
Maximum ion measurements were from different time points, and different techniques were used for metal ion measurements not all of which were the most reliable ICP-MS.
The ion selection threshold for the Orbitrap (MS) was set at 1.0 × 10 with a maximum ion accumulation time of 500 ms.
The total ion current resulting from ESI of the Agilent tuning mix and ions injected to a SLIM linear module was directly measured as a function of position along the ion path.
