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The minimum and maximum intron length was set to 5 bp and 50,000 bp respectively.
A minimum and maximum intron length of 40 and 50,000 were used, respectively.
The maximum intron length allowed by BLAT is 500,000 bases.
We analyzed these low-quality reads, using a minimum intron length of 5 bp, a maximum intron length of 6 kbp, and an anchor size of 6 bp.
HMMSplicer was run with a minimum intron length of 5 bp and a maximum intron length of 80,000 bp, covering 99.1% of known introns in the human genome.
The tRNA gene boundaries and folded structures were defined using tRNAscan-SE (http://lowelab.ucsc.edu/tRNAscan-SE/) [31] with the following parameters: (i) search mode – organellar; (ii) search type – cove only; (iii) cove cut off score −15; (iv) translation code used – invertebrate mitochondrial; (v) maximum intron length – 40; and (vi) pseudogene checking – disabled.
Similar(29)
The gene models derived from MAKER-P yielded maximum intron lengths >50 kbp, with several >100 kbp.
For example, users may want to choose reasonable limits for minimum and maximum intron lengths guided by prior data.
By default, TopHat only examines potential introns longer than 70 bp and shorter than 20 000 bp, but these default minimum and maximum intron lengths can be adjusted by the user.
Filtered reads were mapped to the M. truncatula 4.0 reference genome using Tophat (Trapnell et al. 2009) with maximum indel sizes of 4 bp and minimum and maximum intron lengths of 20 and 2000 bp, respectively.
The maximum allowed intron length was set to 20,000 nt, based on the longest observed intron supported by Sanger ESTs.
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