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Stacks are presented as the maximum intensity Z projections.
To quantify spindle fluorescence, maximum intensity Z projections were first generated using Volocity.
Cell and follicle outlines are usually maximum intensity z projections of ∼2 µm.
Maximum intensity z projections were generated from the merged tile scan image using FIJI software (Schindelin et al. 2012).
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Finally, we created a maximum intensity z-projection (Fig. S1A) and a red-cyan anaglyph of the larva (Fig. S1B).
Are these maximum intensity z-stack projections?
Images represent maximum intensity Z-series projections.
Image represents a maximum intensity Z-series projection.
All images are sums or maximum intensity Z-projections of the relevant confocal slices.
A maximum intensity Z-projection for the anatomy scan and each GCaMP timepoint was used for analysis.
For low magnification, single confocal images, while for high magnification, single confocal images or maximum intensity z-projection (three confocal images at 0.3 μm) images were used.
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