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Glass beads were added, and mixtures were sonicated for 10 pulses of 30 s each at maximum intensity using a Ultrasonic Processor sonicator equipped with a microtip.
Of these, 14 % will have a [M + H]+ < 226 m/ z and will reach only 30%% of their maximum intensity using standard screening settings.
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By means of an input output curve determination conducted before every experiment, the largest obtainable fEPSP was found for each individual animal (maximum intensity used 125 μA).
Then, background signal was excluded determining a threshold over which fluorescent intensity was considered as positive in relation to negative controls, in the maximum slope of intensity using the ImageJ software.
Two experienced observers with more than 2 years of experience in cardiac CT, blinded to the ICA results, independently evaluated all CTCAs for the presence of CAD, using axial source images, multiplanar, curved reformatted reconstructions, and thin-slab maximum intensity projections using "Circulation" software on the proprietary workstation.
Angiograms were obtained by generating maximum intensity projections using Paravision software (Bruker BioSpin GmbH).
Where applicable optical sections were collected at 1 μm spacing and shown as maximum intensity projections using Zen 2009 software (Carl Zeiss).
We remove these by first detecting the contour of the embryo in a maximum intensity projection using Otsu thresholding (Otsu, 1979) and marker-based watershed segmentation (Meyer, 1994), and then discarding all object calls that are outside the convex hull of the detected embryo contour.
The morphology of the small-scale aggregates is also studied by extracting the size distribution from the angle-dependence of the scattered intensity, using the maximum entropy method in conjunction with a simplified tube form factor.
Intensities above 0.6 times the maximum intensity are used to calculate the bright background.
Second, we set the maximum intensity level used for our shaking operators to κ max = 5.
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