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The images were maximum intensity projected (Imaris 6.1, Bitplane, Zürich, Switzerland).
The images were maximum intensity projected (Imaris 6.1, Bitplane, Zurich, Switzerland).
3-D stacks were deconvolved with manufacturer provided OTFs using a constrained iterative algorithm and images were maximum intensity projected.
Each stack was maximum intensity projected, serving as an "autofocus" for the effectively single layer of collagen.
Image files were first processed with ImageJ software, which allowed building a maximum intensity projected image from selected Z-series slices acquired.
The aggregation foci were scored by eye from maximum intensity projected images (spanning the entire volume of the cell) and were defined as puncta that display high-intensity over the surrounding cytoplasmic background signal.
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The images are presented as 3D maxima intensity projected stacks processed with LSM 510 version 4.2 (Zeiss).
Twenty maximum-intensity projected images were used to train each class.
To visualize the 3D datasets, maximum intensity projects were created (MetaMorph, Sunnyvale, CA, USA).
The HRP signal was first rescaled by a factor of 2 and then filtered (Gaussian blur, pixel radius: 2) and z projected (maximum intensity projection).
Maximum intensity projection was used to project developed Z-stacks and files were exported in AVI format.
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