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After a lag of approximately 10 min, ERK phosphorylates eNOS with maximum intensity obtained at about 1 h.
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The average intensity of theR channel was then calculated and normalized to the maximum mean intensity obtained during all the experiments.
After deconvolution, projection images of maximum intensity were obtained and maximum fluorescence intensities over the background intensity used for statistic data analysis.
Coronal, sagittal, and axial WBC slice data were overlaid on the CT slice in color individually windowed so that maximum intensity was obtained in at least one pixel in a given infection focus.
Z-stack images spanning the 2 μm from the surface were acquired, underwent 3D deconvolution using AutoQuant (MediaCybernetics, MD, USA) to reduce the distortion and produce a clearer image, and then flattened using Extended Depth of Field for maximum intensity to obtain a single image (ImagePro Plus, MediaCybernetics, MD, USA).
Figure 1 shows an example of maximum intensity projection images obtained 10, 60 and 120 min after tracer injection.
It has been shown that the resonance depends on the period of grating, and the maximum intensity can be obtained when W = P/2[19].
Maximum intensity projections were obtained in Omero (Version Beta 4.1.1) and exported as Tiff files.
The maximum intensity projections are obtained by displaying the maximum intensity along the depth axis at each transverse position.
Image stacks were captured and maximum intensity projections were obtained using Metamorph 7.1 software (Molecular Devices, Sunnyvale, CA).
The images were deblurred with a no-neighbor algorithm and a maximum intensity projection was obtained from the series of images.
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