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Multiple segmentation algorithms exist, e.g. freehand algorithms (FH), thresholding-based algorithms (e.g. fixed thresholding at 40% of maximum intensity cut-off [40P]), and algorithms based on probabilistic classification of voxels into tumour or background (e.g. fuzzy locally adaptive Bayesian [FLAB]) [9].
λ, optimal shrinkage intensity; qval, cut off for the partial correlation (the threshold is adjusted so as to ensure that all well-studied breast cancer marker genes are included in the networks); α, power-law exponent; Qmax, maximum modularity score; LOO-CV, Leave-one-out cross-validation.
We determined an intensity cut-off of 410 with a 5% false positive rate (see additional file 9).
A signal intensity cut-off could therefore be determined for each array, which reduces these hybridizations with more than 95%.
The very bright SPB signal from Mps3-GFP is excluded from this calculation using an upper intensity cut-off.
Protein abundances exceeding this maximum saturate the detector and are cut off to 100, neglecting the excess abundance and leading to underestimated peak intensities from both the saturated peak and its following peak, as the detector remains saturated for some time [ 12].
An average microarray signal intensity > 20 was used as cut off.
[ 17] We take a cut off point 1/2 (maximum score 12) for indicating poorer psychological health.
Cut off.
molecular weight cut off.
cut off.
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