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Heinz with a maximum insert size of 750 bp (50% deviation), reporting at most 30 hits and removing PCR duplicates.
The only parameter that was varied when mapping was the -i option to specify the maximum insert size.
First, we identified heterozygous germline SNPs that were less than the maximum insert size away from a given mutation on the relevant chromosome (700bp in this sample).
Minimum and maximum insert sizes were set at 150 bp and 350 bp, respectively, to account for variation in insert size within and across samples.
Mapping was done with BWA [ 73] aligning pairs of reads and allowing maximum insert size to 500 bp and keeping only reads with mapping quality higher than 20.
Following ligation of a pair of Solexa adaptors, ligation products with the maximum insert size of 200 bp were gel purified on 2% agarose and subjected to PCR amplification.
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The analysis infers that clad temperature remains within its design limit value even for the maximum inserted reactivity, 2$ at full power operation of reactor.
This study shows that, an unacceptable change of reactor core reactivity was found due to the presence of the HPTL and the maximum inserted reactivity does not exceed 527 pcm at high possible 235U enrichment (10%).
In the case where the "costs" of placing a buffer at different locations are different, we can guarantee to insert maximum number of buffers with minimum total cost.
Due to complex process of actin denaturation and the dependence of the transitions rates upon GdnHCl concentration [11], [12] (see also, Figure 1A, Insert) maximum of ANS fluorescence intensity shifts to lower concentration of GdnHCl with the increase in incubation time.
The largest local deviation of the model from an optimal alpha carbon path does not exceed 6 Å rmsd and reach its maximum in the insert aa192 196, a region that does not inflict on the placement of N-glycans, nor onto the location of cysteine residues in the Cys2 domain (Fig. 1).
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