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The formation temperature, maximum induction temperature and induction time are measured.
Stimulation of c-fos gene expression was dependent on the degree of stretch (maximum induction at 25% elongation).
The background fluorescence and maximum induction level vary significantly from day-to-day, as does the dynamical response of the system to step changes in the light input (Supplementary Note 3).
However, maximum induction of apoptosis in SW-13 cells was observed at 48 h, while only high concentrations of BI-2536 resulted in an increase in apoptosis at early time points (Fig. 5c, d).
The maximum induction appeared in about 20 min. Conversely, the induction process was much slower under low light illumination (10 µmol photons m−2 s−1).
This effect is even more obvious in strain JW49, where at maximum induction only about half of the cells become highly fluorescent.
With multiple operators though the basal level expression is severely repressed, the induction becomes non-linear and the maximum induction is not reached.
Both TCF7L2 and CYP24A1 levels were elevated after 4 hours with TCF7L2 reaching a maximum induction at 24 hours (Figure 3B).
AtHsp90 genes are induced by various abiotic stresses, such as cold, salt, UV-B, etc., although maximum induction is in response to HS (Figure 3C).
While these promoters offer a wide choice of transcription levels, they take ∼15 21 hours to reach maximum induction once thiamine is removed from the medium, and ∼2 4 hours for full repression after thiamine addition.
Unlike the Ca2+ and SA signaling-related genes, maximum induction in Wangshuibai occurred at 12 hai, suggesting that activation of the JA signaling pathway followed activation of the Ca2+ and SA defense pathways.
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