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The maximum imaging depth of the tumor site was measured as 3.5 mm beneath the scalp, which can be seen from both the photoacoustic and the MRI cross-sectional images (Figs. 5a and S9b).
The maximum imaging depth, however, is limited in most tissues to ~1 mm [ 8, 9].
As such, the maximum imaging depth, zmax, must also be increased by increasing the spectral sampling density.
Another limitation of this study was the using of a machine with a maximum imaging depth of 22 cm, low sector width and frame rate.
This method presents important technical difficulties since the maximum imaging depth achievable in the spleen is severely hampered by the absorption and scattering of light by blood.
Only a weak correlation for depth of invasion was observed, as the maximum imaging depth with current technology is limited to 1.5 2.0 mm.
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1– 3 Hinz et al 15 investigated 26 melanocytic skin lesions by means of histopathology, conventional swept-source (1,325 nm center wavelength, 25 μm [lateral] × 12 μm [axial] resolution [air], and maximum theoretical imaging depth of 3 mm [air]) OCT (SS-OCT) (OCS1300SS; Thorlabs®, Dachau, Germany), and 20 MHz HFUS.
In addition, the calibration mirror image limits imaging depth [ 19].
Low-coherence light with a 1310 nm center wavelength and 90 nm full width at half maximum (FWHM) was used, and imaging depth and depth resolution were 3.4 mm and 8 µm in air, respectively.
As seen from Fig. 2(b), the axial resolutions of both systems are almost identical before the 2.5 mm depth, which is about the maximum imaging range of the SU1024 system.
This imaging depth was achieved with a laser fluence on the tissue surface of 20 mJ/cm2, which is below the maximum permissible exposure (MPE) of 25 mJ/cm2 recommended by the American National Standards Institute (ANSI).
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