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Amongst the Indian and Bangladesh sequences, the isolate Ck/Bangladesh/364/07 showed maximum identity with a Saudi Arabia isolate Hb/SA/67321/07 and Kuwait isolate Ck/KT/KISR2/07.
Stretches of amino acids spanning the S1 RNA binding domain display 33% - 73% maximum identity with gaps, depending on the species comparisons.
Since the identified MAPK gene shows maximum identity with tobacco WIPK and Arabidopsis AtMPK3 (Additional file 2: Figure 2) we named it as CrMPK3 (EF156758).
The combined β-tubulin and calmodulin gene sequences of the 99 isolates also showed maximum identity with sequence from reference A. flavus (CBS100927) strain.
The sequencing of positive plaques obtained after tertiary screening gave a 630 bp fragment that showed maximum identity with Wound Induced Protein Kinase (WIPK) from Nicotiana attenuata (ABJ89813).
Custom Perl scripts enabled contigs to be grouped based on their maximum identity with either A or B genome reference sequences.
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Based on the BLAST search, presented in Table 1, the CS sequence of isolated B. bassiana showed maximum identity (100%) with fungal strain CCFC225554 (accession no: AY283555.1).
Of the R. parkeri positive samples sequenced, maximum identity was seen with R. parkeri sequences (GenBank accession no. FJ986616.1).
The nucleotide sequence of ompA was closest (99.8% identity) to that of the ompA of Rickettsia sp. strain Davousti, and showed maximum identity (97.2%) with R. heilogjiangensis as validated species.
PknJ kinase domain shows considerable identity with other Mycobacterium STPKs (including maximum identity of 50% with PknF).
Domain prediction revealed a TIR structure between 1 and 87 amino acids that had a maximum identity of 58%and7676% with TLR2 – TIR protein of teleost fishes and higher mammals respectively.
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