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Domain prediction revealed a TIR structure between 1 and 87 amino acids that had a maximum identity of 58%and7676% with TLR2 – TIR protein of teleost fishes and higher mammals respectively.
PknJ kinase domain shows considerable identity with other Mycobacterium STPKs (including maximum identity of 50% with PknF).
A transposed summary also was created to evaluate the maximum identity of all pseudogenes against each given expression cassette, to capture the likelihood that any given cassette could have sampled from particular vs. multiple pseudogenes.
Another 16 clones were closely related to N. euthropha C91 with a maximum identity of 99%.
The smaller RNase P family has 28 training sequences and 15 testing sequences, with maximum identity of 60%.
The partial 16S rRNA gene (1309 bp) from the strain ME27-1 showed a maximum identity of 99% with that of Paenibacillus terrae AM141T (T: type strain).
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A Spearman rank correlation coefficient was calculated in order to assess whether maximum identities of pseudogenes near the expression site were higher than for those more distantly positioned pseudogenes.
Isolates with the maximum scores and maximum identities of 100 or 99% and documented as approved nomenclature were accepted for species identification [ 23].
Using ranked correlation, genomic distance from the expression cassette accounted for 21% of the variance in the ranking of maximum identity to some expression cassette (P = 2.4×10−6).
The maximum identity for each pseudogene against all possible expression cassettes was determined and the mean, standard deviation, mode, and range of maximum identities calculated.
Only related sequences with a maximum identity percentage of 90 were used for this analysis.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com