Based on the BLAST search, presented in Table 1, the CS sequence of isolated B. bassiana showed maximum identity (100%) with fungal strain CCFC225554 (accession no: AY283555.1).
Of the R. parkeri positive samples sequenced, maximum identity was seen with R. parkeri sequences (GenBank accession no. FJ986616.1).
Further, all the inserts were also similar to the gene encoding the type IV B pilus protein in P. aeruginosa (accession no. YP_792920), with 81% coverage and 95% maximum identity.
However, all the inserts from 2 PCR amplicons were highly similar to the gene encoding the putative PilL protein found in P. aeruginosa (accession no. ABR13432), with 82% coverage and 97% maximum identity by blastx search.
The deduced amino acid sequence of phaR exhibited high identity to B. vietnamiensis (100% identity, accession no. YP_001119554.1) and C. necator (83% identity, accession no. YP_725943.1).
The putative phaB Bs exhibited significant identity to acetoacetyl-CoA redutase from B. vietnamiensis (99% identity, accession no. YP_001119555.1), P. putida (92% identity, accession no. BAB96554.1) and C. necator (89% identity, accession no. YP_725942.1).
The deduced amino acid sequence of phaC Bs exhibited great similarity to PhaC of B. vietnamiensis (99% identity, accession no. YP_001119557.1), Pseudomonas putida (73% identity, accession no. BAB96552.1) and Cupriavidus necator (63% identity, accession no. YP_725940.1).
The deduced amino acid sequences of putative phaA Bs were similar to those β-ketothiolase from B. vietnamiensis (99% identity, accession no. YP_001119556.1), P. putida (90% identity, accession no. BAB96553.1) and C. necator (87% identity, accession no. YP_725941.1).
The closest hit when analysed by BLASTn was Naemacyclus minor (90% sequence identity, accession number KJ606680).
OTUP34O 3 with 10 sequences was identified as closely related with the P34O genes contained in Martelella mediterranea (89% identity, accession number: WP_018063819); and OTUP34O 4 with 6 sequences was mostly identified with Thalassospira lucentensis (100% identity, accession number: WP_062953075).
