Based on maximum identity score and query coverage, the best highly identical 10 sequences were selected and aligned using multiple alignment software program, ClustalW (MEGA tool).
The maximum identity accession has been found and the structure was analyzed.
Based on the BLAST search, presented in Table 1, the CS sequence of isolated B. bassiana showed maximum identity (100%) with fungal strain CCFC225554 (accession no: AY283555.1).
Based on a maximum identity score achieved from BLAST, strains, having exactly same or nearly same sequence across the D2 region in 28S rDNA, were selected.
Sequence-based identities with the best hit were defined as the sequences with the highest maximum identity to the query sequence.
b: Maximum identity, query coverage, and taxonomic information about the species of fungi from the patches invaded and un-invaded by Conyza sumatrensis.
b: Maximum identity, query coverage, and taxonomic information about the species of bacteria from the patches invaded and un-invaded by Conyza sumatrensis.
Domain prediction revealed a TIR structure between 1 and 87 amino acids that had a maximum identity of 58%and7676% with TLR2 – TIR protein of teleost fishes and higher mammals respectively.
With a view to identify the isolated B. bassiana sp., a BLAST search was carried out between CS and NCBI database and 15 sequences, with their respective host organisms, were selected based on maximum identity score (Table 1).
The mean maximum identity across all pseudogenes was 80.4% (21.4 s.d)., and the mode was 100%.
PknJ kinase domain shows considerable identity with other Mycobacterium STPKs (including maximum identity of 50% with PknF).
