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At time 0 (the, the average increase in size in EMEM/S- was 86 ± 21 nm, similar to the distribution of most PBH-capped NPs in EMEM/S+, except Au[(Met)2B], which experienced extensive agglomeration at time 0 (1,568 nm) with smaller fluctuations in its maximum hydrodynamic diameter over 24 h in EMEM/S- (1,368 nm).
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Maximum intensity hydrodynamic diameter (nm) measured directly after preparation (T0) and at 2 h (T2), 4 h (T4), 24 h (T24) and 48 h (T48) of incubation are shown.
Au[(Gly-Tyr-TrCys)2B] was the only preparation of AuNP that remained in the same relative size distribution profile over time and with the same maximum intensity hydrodynamic diameter (±54 nm) after a 24-h incubation in EMEM/S-.
In water, all AuNP preparations formed agglomerates, showing characteristic maximum intensity hydrodynamic diameters of ≤200 nm (Table 2).
For the obtained Ce0.85Tb0.15F3 NP, intensity distribution of the hydrodynamic diameter gave main maximum at 62 ± 36 nm (about 97% of intensity) with negligibly small addition of larger fractions.
Comparison of Figs. 1 and 2 shows that the size of the nanoparticles obtained by TEM is smaller than the hydrodynamic diameter at the maximum of DLS intensity distribution.
The mean values ± S.D. of the hydrodynamic diameter and the polydispersity index (P.I). are reported.
In neutral environment (pH 7) the hydrodynamic diameter of the NCs was 407 nm.
(b) Formation of the nanoporous particles and the changes of hydrodynamic diameter measured by DLS.
The average zeta potential of NDs in water and hydrodynamic diameter of NDs were obtained by DLS (Delsa Nano, BECKMAN COULTER).
When the pH was adjusted to 10, the hydrodynamic diameter of the NCs was found 514 nm.
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