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A line was drawn from the meniscus to the bottom of the culture tube in each image and used to measure the maximum gray intensity and the plot profile of intensity along the line.
From the plot profile data, the x-coordinate along the line at which half of the maximum gray was reached was calculated and used to calculate a ratio: x coordinate of half of maximum gray intensity / length of the line.
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The first step of conversion processes is to normalize the L number of samples so that their values are between 0 and maximum gray-scale pixel intensity.
To further test the hypothesis that the OSN encodes features related to the slope of the stimulus, we examined a sigmoid ramp for which the first derivative of the stimulus reached its maximum (gray arrow) prior to the stimulus intensity (magenta arrow).
The selected mean value of gray intensity is 127, between the minimum 0 and maximum 255 of each pixel.
Pixel intensity in the entire ASJ cell body was determined from captured images in the form of maximum gray values by using NIH ImageJ software.
where N g is the maximum gray level and R max is the maximum length.
The mean value of the gray intensity for each extracted pixels group is computed.
First, the gray intensity of each individual image was examined to ensure an equal distribution of gray.
Specifically, the amount of protein was estimated as maximum gray value for each protein band.
In addition, although the model was deduced on the condition that the mean intensity is low compared to the highest intensity the CCD can record, the result in Fig. 9 indicates that the model appears to work even when the mean gray level is more than half of the maximum gray level for a 12 bit CCD.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com