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A maximum genetic distance of 3% is sufficient to distinguish all the catfish in this study.
The greatest genetic distance between any pair of cattle-derived isolates was 24.95%, which is 10-fold greater than the maximum genetic distance observed between any pair of Tp1 sequences from cattle.
The maximum genetic distance between the corresponding viruses was 13 nt (2.9%).
These subgroups are separated by a mean, minimal, and maximum genetic distance of 2.28%, 1.71%, and 2.89%, respectively.
The maximum genetic distance was 24 nt (5.3%), and the minimum distance between 2 groups was 11 nt (2.4%).
We assumed that the maximum genetic distance at synonymous sites roughly corresponded to the TMRCA of DQBs.
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Initially, the pairwise comparisons with maximum genetic distances observed among the 117.370 possible combinations were ranked.
We then used the codeml package from PAML (v.4.9a) on each of these alignments with the WAG amino acid rate matrix to calculate maximum likelihood genetic distances between the two sequences40.
Maximum uncorrected genetic distances from the Kikihia dataset are similar to those in Amphipsalta Notopsalta.
Maximum patristic genetic distances between haplotypes from the partitioned ML analysis were 0.005 substitutions/site (s/s) in A. cingulata (0.004 uncorrected, or p distance) and 0.011 s/s (0.009 uncorrected) in A. zelandica.
Branch support values for the 8 major clusters obtained in ML and Bayesian analysis can be seen in Table 1, together with maximum pairwise genetic distances (a criterion not employed for cluster identification).
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