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To avoid exceeding the maximum gene list size allowed by IPA program, we limited targets based on each programs assigned score.
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In fact, the differences in performance when assessed on candidate lists consisting of 100, 200 or 500 candidates are minor and do not suggest that the maximum candidate gene list size will be encountered in typical gene mapping experiments.
Permutation analysis yielded a maximum intersection gene list size of 10 genes only 3 times in 100,000 trials inferring a p-value close to zero for the 234 genes found in common to the regional metagenes listed in Additional File 1.
Due to the calculation complexity and limitation of the FACTA+ system, the input file should contain maximum 7 different gene lists with maximum 100 genes in each list.
The app provides access to most of the features of the GeneMANIA prediction server while removing limitations on gene list length, and the maximum size of the resulting network.
Thus, the maximum possible fraction of overlap between our grain filling gene list and the control set could be 100%.
Sorting the gene list on number of samples implicated and maximum Phylop scores for placental mammals in the found variants, SETBP1 ranked at the top of the list as the prime candidate (Table 3).
Therefore, in order to concentrate on the biologically more meaningful pathway collections, an arbitrary cut-off of a maximum of 150 genes per pathway was chosen as the largest reported gene list.
Such 'leading edge genes' were defined as subsets of genes that appappeared in an expression ranked gene list before the enrichment score of a given pathway reached its maximum [ 27].
As the cumulative length of the predicted exons exceeded the available baits, following bait design in eArray we looped through the gene list, selecting one bait for each gene at each pass, until the maximum number of baits was reached.
We also analyzed the biological functions of several top genes within the 19 gene list.
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CEO of Professional Science Editing for Scientists @ prosciediting.com