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Using this model, we found that the probability of obtaining maximum gene diversity under a random sampling scheme is extremely small compared to directed sampling (P<0.0001).
The number of alleles per locus ranged from two to 23, and the maximum gene diversity was 0.846 (Table 2).
In the same way, the estimates of molecular diversity (maximum gene diversity, H, and nucleotide diversity, π) were higher for COI than for rbcL, regardless of the haplogroup considered (Table 1).
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With prolonged development, the transcriptional program of iTregs diminished steadily, resulting in about three times lower numbers of genes expressed in iTregs as compared to Teffs at day 10, whereas the gene diversity between the two populations achieved its maximum.
These two steps were repeated until the gene diversity D of the subset reached a maximum.
SNP markers are mainly biallelic, the gene diversity and PIC thus cannot exceed 0.50, whereas the maximum can approach 1 for multi-allelic markers, such as SSRs.
Taking into account that the maximum level of expected heterozygosity for biallelic markers is 0.5, a high degree of gene diversity was found for the 25 SNPs selected in all the populations analyzed.
Genetic diversity was investigated by computing the proportion of polymorphic loci (PPL) at the 5% level and Nei's gene diversity (Hj, analogous to He) using standardized sample sizes (i.e. with a maximum of eight samples per population).
In addition, the laccase gene diversity was determined.
The gene diversity reported in our study is lesser than gene diversity (0.68) reported by (Liakat Ali et al. 2011).
The possibility of gene diversity was checked by PCR with primers specific for WsA mesoglein gene.
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