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The maximum gap length was 5 bp, and at least three pairs of reads to define an indel.
Thus, the number of elements in the multidimensional table is never more than the sequence length times the maximum gap length raised to the interleaving number power.
Minimum overlap length was set to 100 bp, minimum identity percent to 97% and maximum gap length to 30 bp (-o 100 -p 97 -f 30).
Then, at every split state, the number of dimensions of the dynamic program will be multiplied by the maximum gap length, because the dynamic program must keep track of scores for each possible sequence position (up to the maximum gap length) that could be mapped to that state.
For these sequences we have performed closing of gaps (5 gaps, maximum gap length of 7 bp) and confirmation of correct assembly with PCR and Sanger sequencing (total 5700 bp).
A whole genome alignment was performed with Mugsy [ 35] on a total of 23 available A. baumannii genomes (Additional File 2, Table S2) using a maximum gap length between orthologous blocks of 100 base pairs.
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Table 3 shows the percentages of both types of errors for the three tests of consistency described above for the five maximum gap lengths.
In other words, these two parameters define the maximum allowable gap length, both horizontally and vertically.
To investigate whether the set of assembled sequences contain alternative transcripts of the same loci, the contigs and singlets were further clustered using the SeqManII software with the following relaxed parameters: match size = 12; maximum added gap length = 70; minimum percent match = 80; no gap penalty; gap length penalty = 0.70.
(c) For longitudinal gap, the two vehicles have to be in the same frame and hence the maximum gap is equal to trap length.
Single-nucleotide polymorphism (SNP) candidates were screened using the CLC Genomics Workbench 5.1 with the following parameters: window length, 11; maximum gap and mismatch count, 3; minimum coverage, 1; minimum variant frequency, 75%.
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