The statistical report section, including minimum, maximum, average, standard deviation and the maximum fold change of the expression levels in the selected libraries, also provides support to investigate specificities for the desired query set.
In addition, a complementary set of statistics, such as the minimum, maximum, average, standard deviation and maximum fold change of the expression levels of each locus in the selected libraries are provided.
As a result, a list of 15 genes was suggested with the most constant expression level, based on a coefficient of variation smaller than 4%, a maximum fold change smaller than 2 and a mean expression level lower than the maximum expression level minus 2 times the standard deviation.
The difference between the fold up-regulation of dfmr1 mRNA (∼1.5×) compared with the maximum fold up-regulation of dFMR1 protein levels (2 4×) in the Ago2 null strain suggests that dfmr1 translational efficiency may be affected by the loss of Ago2.
Gene expression levels, expressed as "relative fold change", were determined using a method comparable to the 2-ΔΔCt method described by Pfaffl [ 16], where the point of peak cycling acceleration was extrapolated to represent maximum fold change.
Of note, not only the transcriptional regulation of RELB (maximum fold change of 7.4) was more significant than that of RELA (maximum fold change of 6.5), but also RELB had higher transcriptional levels than RELA.
For example, a 20-fold increase in expression level at 5 copies/ng (ERCC-51) is associated with 16-fold and 10-fold difference in the minimum and maximum fold changes detected by the BioMark arrays and ABI 7900HT system respectively.
This involved plotting the minimum positive dose level (expressed as mumole/kilogram) and the maximum fold-increase in micronucleated polychromatic erythrocytes frequency observed at any dose level.
The data (0 to 3 folds) were displayed and clustered by MeV v4.8 software (http://www.tm4.org/), and the genes induced by each stress with their maximum folds (> 2 folds) of relative expression level were accounted and performed in the Figure 7.
At 96 hr post infection the maximum numbers of total genes were upregulated at ≥ 1.5 fold levels in mouse brain.
