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These models enumerate the biochemical reactions involved and impose mass balance, thermodynamic and maximum flux constraints to define the set of flux vectors representing all possible steady states.
E-Flux treats the production of such metabolites as an artificial biomass function, and uses FBA with maximum flux constraints to calculate the maximum value of this function.
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In additions, while E-Flux applied hard constraints on maximum flux, E-Flux-MFC borrows a key idea from the PROM method [ 49] and allows fluxes that violate the maximum flux constraint, but penalizes such violations.
It operates directly with reaction constraints setting the maximum flux capacity in accordance with the gene expression level.
In a next step, it will be interesting to directly incorporate the gene expression information in the model, providing additional constraints on the maximum flux [ 23, 24].
The size of this space can be reduced by incorporating thermodynamic constraints associated with irreversible reactions, as well as flux capacity constraints, which limit the maximum flux through some or all reactions.
The space of allowable solutions can be reduced by incorporating thermodynamic constraints associated with irreversible reactions, as well as flux capacity constraints which limit the maximum flux through certain reactions.
The temperature rise, efficiency, regulation and maximum flux density in stator teeth are taken as constraints for design problem.
The files R, N, and C can then be supplied to the FBA Constraint Variation-Objective Function Analysis process to determine the maximum fluxes of the reactions in R that are denoted in N for each set of flux constraints in C. Each of these files can be edited by the user at any step, and many other combinations of processes are possible.
Flux constraints are rarely, if ever, applied.
Equilibria are determined, in part, by magnetic flux constraints.
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