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However, in Table 2, small variations of wavelength-independent maximum fluorescence are observed for oil-in-water suspensions.
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Maximum fluorescence was observed after just 1 h of incubation, indicating fast cell uptake of FITC-AmB-MWNTs.
When 34 µM tubulin alone was incubated at 37°C, a rapid increase in DAPI fluorescence was observed due to the preferential binding of DAPI to assembled microtubules and maximum fluorescence was observed in approximately 45 minutes.
The fluorescence increase in ST was slower and the maximum fluorescence was less and appeared later than in TT.
Measured hours were selected as the best times for different experimental conditions (maximum fluorescence was detectable at 24 h).
Existence of an optimal concentration resulting in a maximum fluorescence was established to obtain optimal lipid staining for both dyes.
Annexin-V maximum fluorescence was detectable at 3 h with 24% early apoptotic cells (annexin-V+, PI−) and 40% late apoptotic cells (annexin-V+, PI+).
Duysens and Sweers [ 40] postulated in 1963 that the fluorescence changes reflect primarily changes in the redox state of QA, in a way that the maximum fluorescence is reach when the pool of QA is completely reduced [ 29].
Maximum fluorescence was observed at 568 nm (in a rather broad peak, excitation 440 – 460 nm), consistent with the microscopic visualization of the green and yellow fluorescence with an FITC filter [ 16].
Liposomes were burst at the end of the experiment by the addition of 1 μl 0.2M C12E8 detergent (Sigma-Aldrich), and the maximum fluorescence was found by monitoring the sample for an additional 10 min.
We truncated the trajectories of the time courses when the maximum fluorescence was reached as our previous work showed that reporter signal is diluted and distributed equally into daughter cells after cell division (Downey et al. 2011).
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