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This allows to convert the % of maximum fluorescence (achieved by washing on lactate only) into NADH/NAD + ratios.
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This indicates that maximum fluorescence intensity is achieved at sparse labeling with ∼30 dyes per particle; as the dye density increases, fluorescence quenching occurs, reducing the overall emission intensity.
To achieve maximum fluorescence heads were visualized using the following emission filters: LP650 BP565-615 BP 565-615.
maximum fluorescence intensity.
Chlorophyll fluorescence measurements included initial fluorescence (Fo), maximum fluorescence (Fm) and variable fluorescence (Fv).
the maximum fluorescence at the steady state.
Fluorescence spectra are normalized to the maximum fluorescence values at 690 nm.
Responses of the reaction systems were the maximum absorbance (Y1) and maximum fluorescence intensity (Y2), correspondingly.
Electron transport rate (ETR), and the ratio of variable fluorescence to maximum fluorescence (Fv/Fm) for leaf samples were measured using PAM-210 fluorescence meter (Walz, Effeltrich, Germany).
The maximum fluorescence in the light-saturated stage (F′m), basic fluorescence after induction (F′0), and fluorescence yield in the steady state (Fs) were determined.
GdBN exhibited the maximum fluorescence emission at λem = 440 nm and the maximum excitation at λexc = 360 nm. Figure 1 Fluorescence spectra of label free GdBN.
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