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The focussed kinase library of compounds was solubilised in DMSO with a maximum final assay DMSO concentration of 1%% (v/ v).
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The final assay volume was 200 μL.
The final assay protocol was as follows.
The final assay volume was 2.0 mL.
The final assay volume for both assays was 2.0 mL.
The maximum final concentration of DMSO was 0.1% (v/v).
A final assay concentration of 40 nM SaFBA was used.
The final assay protocol is summarized in the Table.
The Dundee Drug Discovery Unit (DDU) diversity set of 59,904 compounds was solubilised in DMSO (final maximum assay concentration of 1% (v/v) in all samples including controls) and a HTS performed in singlet at a concentration of 30 μM.
Values from the final two assays were regressed to the original assay.
DMSO concentrations in final cell assays were less than 0.01%.
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