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To search for the optimal channel design for maximum fill time reduction, the Medial Axis (MA) of the part surface is defined.
Each full scan consisted of 1 microscan with a maximum fill time of 50 milliseconds; each MS/MS scan consisted of 1 microscan with a maximum fill time of 100 milliseconds.
Ion target values were 1,000,000 (or maximum fill time of 10 ms) for the survey scan and 10,000 (or maximum fill time of 100 ms) for the MS scan, respectively.
Ion target values were 1,000,000 (or 500 ms maximum fill time) for full scans and 10,000 (or 50 ms maximum fill time) for MS/MS scans.
The automatic gain control (AGC) target was 2 −10 × 10 ion counts with maximum fill time of 2 s.
Automatic gain control (AGC) was used to accumulate sufficient precursor ions (target value 1 × 10 charges, maximum fill time 100 ms).
Similar(46)
Maximum fill times were set to 1,000 ms for full MS scans and 500 ms for MS/MS scans with minimum MS/MS triggering thresholds of 5,000 counts.
Maximum fill times were set to 1000 ms for full MS scans acquired in the OT and 250 ms for MS/MS acquired in the linear ion trap, with a CID energy setting of 35% and a dynamic exclusion of 60 s for previously fragmented precursor ions.
We observed that effective pacing can be ensured with pacing at short AV intervals; however, it has been shown that the optimal AV delay during CRT targets maximum filling time.
Both MS and MS/MS spectra were acquired using one microscan with a maximum fill-time of 1000 and 100 ms for MS and MS/MS analysis, respectively.
The target value for the Orbitrap was 10 charges and the maximum allowed fill time was 100 ms.
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