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These results confirmed the predictability of the models in determining the optimum conditions needed to obtain the highest antioxidant activity and maximum extraction yields of the C. nutans leaves extracts (Fig. 1).

The objective of this study is to optimize the extraction conditions (extraction temperature, extraction time, and solvent ratio) needed to extract the phenolic components in C. nutans leaves and to determine the optimum conditions for the maximum extraction yields and the highest anti-radical activity of the extracts.

Optimized conditions for the simultaneous maximum extraction yields, DPPH radical scavenging activity and TPC were determined.

The results showed that solid liquid ratio plays a significant role in attaining maximum extraction yields of baicalin.

The maximum extraction yields of prunetin, tectorigenin, genistein and biochanin A reached 1.204, 1.057, 0.911 and 2.448 mg/g dry weight, respectively.

The maximum extraction yields of genistein and genistin reached 0.418 and 0.398 mg/g, respectively, under the optimal conditions: extraction time 45 min, particle size 50 mesh, negative pressure −0.05 MPa, ethanol concentration 70% and liquid/solid ratio 44:1.

The maximum extraction yield of acetic acid achieved 71.7% at pH 1.02, 31.4 °C, 46.8 min, and 1.07 times.

Maximum extraction yield (34.60% (dry basis)) was obtained at 500 bar, 60 °C and flow rate of 15 g/m.

Experiments were conducted to evaluate the effects of four independent variables on the maximum extraction yield of polysaccharides.

Optimisation of the reaction parameters for maximum extraction yield of PEM was carried out using response surface methodology.

The maximum extraction yield of 55 mg/g was obtained at extraction time 5 min, solid to solvent ratio 1 20 and microwave power of 272 W.