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These transcripts displayed a transient response with the maximum expression reached between 12 and 24 h.
However, with these modifications, the maximum expression reached only 15±2%2% relative to Pcpt.
We adapted the tissue specificity index, τ. to investigate how narrow Nups network genes expression is across development: where N is the number of stages being compared, E i is the expression in stage i, and Emax is the maximum expression reached by the gene across stages (Yanai et al. 2005).
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We have previously demonstrated that some defense-related genes start to increase their expression at 24 hpi, but maximum expression was reached at 48 hpi [ 12].
Figure 2A, lane 3 shows that maximum expression was reached by incubation of auto-induced cultures for 24 h at 30°C.
Induced protein levels of MMP-1, MMP-3, and MMP-10 were detected by 8 h and maximum expression was reached by 24 h posttreatment (Fig. 3E).
As expected, WAP (and Cre) expression reached maximum levels during lactation.
Control neurons had low or negative Bcl-2 signals, in agreement with a previous report, whereas Bcl-2 labeling was stronger in HD neurons, and Bcl-2 expression reached maximum in the brains of HD patients with longer disease duration.
CYP1A1 and CYP1A2 mRNA expression increased with flow rate and reached a maximum after 15 and 7 days, respectively (p<0.01), while CYP2B6, CYP3A4 and GSTα mRNA expression reached a maximum at 250 μL/min and decreased thereafter, maximum expression requiring at least 4 days of exposure to flow.
In this experiment, the EGFP protein expression reached the maximum value at 72 h.
Its expression reached its maximum on day 14 when cultured on a polystyrene control, while it was cyclically up-regulated on Ny-t.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com