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Crocidolite induced the expression of all three proteins with a maximum expression after approximately 18 hr in fresh media.
Triggering of TLR3 with polyI C had resulted in significantly increased ICAM-1 expression (SS-SGEC: sixfold, controls: sevenfold) that reached maximum expression after 24-hour stimulation.
Yet, consistent with the mRNA analysis, NDRG1 protein was detected after 2 h of hypoxia and its level steadily increased reaching a maximum expression after 48 h in Capan-1 cells.
Interestingly, under these conditions, the IFNα-induced expression of OAS2 (in hST2-U3C) and Ifit1 (in mST2-MS1KO) dramatincreasedcreased as compared with the control cells (Migr1-U3C and Migr1-MS1KO, respectively), with a maximum expression after 24 h of IFNα treatment.
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Each member of this gene family exhibited a similar expression profile upon exposure to low temperature with maximum expression observed after 2 hours.
Up-regulation of the mussel lysozyme, with increased percentage of hemocytes expressing lysozyme mRNA, was observed at 2-3 days post-injection of Vibrio anguillarum or Micrococcus lysodeikticus [ 88] whereas maximum expression occurred after 3 hours in hemocytes immunostimulated in vitro [ 75].
The expression of FREPs agrees with results reported in the mussel after a bacterial infection in which the maximum expression was obtained after 6 hours post-infection [ 19].
Maximum expression was observed after 4 days of methanol induction at 30 °C.
Under 2.5% O2, AngII protein levels increased and reached a peak 1 h later (earlier than the maximum expression of myocardin after 4 h) before declining gradually thereafter.
Maximum expression of CsLOX1 after the MeJA treatment was at 12 h and decreased thereafter, although it remained higher than that in the control at 48 h.
The diurnal expression analysis of rice and Arabidopsis MADS box and seed storage protein coding genes listed in the networks revealed that the MADS box genes fall in distinct groups – some with a phase of 12 (i.e., maximum expression at 12 hours after lights on) and an end-of-day crest and another group cresting in the dark or at dawn (Additional file 6).
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