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The predicted maximum ethanol generation rate was 2.37 g L−1 h−1.
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However, public pressure on food vs. fuel and the 10% maximum ethanol blend wall are moving biofuel investment toward next generation lignocellulosic biofuels [2].
Specifically, ethanol producers are asking that the maximum ethanol content in the most common blend of gasoline be increased from 10percentt — a limit set about three decades ago — to as much as 15percentt.
However, the maximum ethanol that could be produced was 43.5 gL−1.
Maximum ethanol selectivity was 5.5%, measured as carbon efficiency.
Ethanol concentration reached its maximum (1.1 g.L−1) after 48 h, corresponding to a maximum ethanol yield (Y E/S ) of 0.20 ± 0.00 g ethanol.g−1 substrate and maximum ethanol productivity (P E ) of 27.6 ± 0.0 mg ethanol. L.h)−1 (Table 1).
Maximum ethanol production obtained during BBD was 15.6 g/L with ethanol productivities 0.43 g/L/h.
Regression analysis of experimental results shows that all process parameters had significant role on maximum ethanol production, glucose solubility, ethanol yield and ethanol volumetric productivity.
In figure 1A, the maximum ethanol concentration (for EE2 1 µM) is less than 0.01%.
However, maximum ethanol production was not reached until 216 h.
The maximum ethanol titer reached 56.3 g/L.
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