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The maximum ethanol flux of 1.71 was obtained by modulating HXT and TDH.
Table 1 lists the maximum ethanol flux ratio and modulated enzymes using different allowable numbers of manipulated enzymes.
For each alternative media formulation, FVA was performed for the ethanol exchange flux and the maximum ethanol flux was used along with the predicted maximum growth rate to calculate the ethanol:biomass yield.
The Pareto front of the resilience problem is identical to the result obtained by MIHDE, as shown in the green data curve of Figure 2. The maximum ethanol flux ratio for different allowable numbers of manipulated enzymes fell to 60-70%.
Similar(55)
In the wild-type seen in Figure 6-A, optimal growth can occur across a wide range of ethanol flux values, limited by the maximum glucose uptake rate.
Dynamic metabolic flux analysis clearly shows that the fluxes from pyruvate to lactate and formate are both strictly constrained throughout the process and that both the maximum ethanol yield (0.46C/C) and the maximum specific productivity (19.8 mmol C (g DCW −1 h−1) occur at late-exponential growth phase.
However, the maximum ethanol that could be produced was 43.5 gL−1.
The predicted maximum ethanol generation rate was 2.37 g L−1 h−1.
Maximum ethanol selectivity was 5.5%, measured as carbon efficiency.
Water and ethanol fluxes were successfully fitted to an adsorption solution model derived from the Maxwell Stefan theory, where ethanol flux is coupled with water flux.
The addition of NaCl improved the membrane selectivity and ethanol flux but slightly lowered down total permeation flux.
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