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Maximum enzyme production (116 ± 3.45 U l−1) was achieved in submerged fermentation.
It predicts the maximum enzyme production among the selected range of variables and also studies the interaction among independent variables.
The fermentation time for the maximum enzyme production is dependent upon the organism type and cultural/environmental conditions.
Similar result has been reported by Ravikumar et al. (2012) with maximum enzyme production at pH 5.0.
Maximum enzyme production (5.36 IU mL−1) was obtained at 27 °C at 120 h and the production declined significantly (0.87 IU mL−1) at 30 °C.
The maximum enzyme production was also found at moderate pH by others that support the present study (Matikeviciene et al. 2011; Kim et al. 2001).
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There was a reduction in pH from 6.5 to 3.0 during intra- and extracellular inulinase and invertase productions, and maximum enzymes production was observed at pH 4.6 (data not shown).
In the present study, several filamentous fungi were screened for production of amylases and among them Aspergillus fumigatus KIBGE-IB33 was selected based on maximum enzyme yield.
The maximum gelatinolytic enzyme production was achieved (~2,800 U/mL) at the high gelatin concentration (~8 g/L) with the cultivation time of 30 32 h.
Three-dimensional response surfaces were plotted on the basis of the model equation to investigate the interaction among the various variables and to determine the optimum concentration of each factor for the maximum fibrinolytic enzyme production– 2(c)).
Yeast extract as organic N2 source and potassium nitrate as inorganic N2 sources gave the maximum amount of enzyme production.
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