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The maximum drug release was obtained at 5.16 of pH and 107 h of release time.
The maximum drug release rate (3.55%h−1) was from the microemulsion M3 comprising 16%, w/w of poloxamer 407.
These results clearly show that the maximum drug release in all three formulations was 96%% of the total drug but rate and style of release was different.
Thereafter, release rate of DTX from DTX-NLC formulation was faster up to 35 h when the maximum drug release (96%%) was reached while in case of DTX-NLC in gel, the same rate of drug release was continued up to the maximum of 96%% in about 53 h.
FPRT of optimized batch F13 showed maximum drug release 99.89 ± 2.01 and less buoyancy lag time 102 sec.
Optimized FPRT batch F8 showed lag time of 7 ± 0.1 h and 55 ± 2 sec floating lag time along with maximum drug release (98.69 ± 2%).
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The maximum cumulative drug release of 81% was observed over a time period of 250 h at pH of 4.4.
Solution layering process resulted with high drug content (81 94.2%) and maximum drug was released by end of 12 h with 6 h lag phase.
Maximum in vitro drug release was found to be 94.78% in 6.0-h study.
The in vitro release studies were performed in 0.1N HCl for 1.5 h, followed by pH 6.8 phosphate buffer for 2 h and pH 7.4 phosphate buffer till maximum amount of drug release.
The results presented in figure 2B show that the particles formed both by direct-nano and conj-nano were sensitive below pH 6, maximum amount of drug release at pH 5 suggesting that a pH dependent release of drug can take place from the particles.
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