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An estimated volume for each metastasis was calculated from the maximum diameter measured at the pleural surface.
Arteriole diameter in the figures is expressed as percentage of the maximum diameter, measured in 0 mM calcium, 2 mM EGTA containing Kreb's solution.
Arterioles were equilibrated for 60 min, until pressure-induced constriction (myogenic tone) developed, usually about 50% of the maximum diameter measured in calcium free Krebs (2 mM, EGTA) and the diameter (μm) was measured by video microscopy using either an automated computer program (DiamTrak) or video callipers as previously described [ 33].
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Lateral root spread together with canopy spread, was taken as the average of the maximum diameters measured by tape measure in two directions at right angles, approximating a N-S and E-W line.
Cardiothoracic ratios were calculated from digital chest radiographs (ratio between the maximum cardiac transverse diameter and the maximum thoracic diameter measured between the inner margins of the ribs).
For each tree the ratio of maximum branch diameter measured to the mean of measured branch diameters was calculated.
To simplify the calculations in this study, the maximum tumor diameter measured was that of the primary tumor.
Mann-Whitney test was performed to compare maximum tumour diameter (measured as a continuous variable) between groups of independent samples.
Tumor size was defined as the maximum tumor diameter measured on the tumor specimens at the time of surgery.
Tumor size was defined as the maximum tumor diameter measured on the mammogram and/or ultrasonogram at the time of diagnosis.
When the maximum tumor diameter measured approximately 0.5 cm, each mouse was treated with a total of 5 doses of MV-CEA (1.0 × 10 TCID50) or an equivalent dose of UV-inactivated MV-CEA for 10 days (defined as untreated, n = 8 per group).
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