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Protein bands were visualized with chemiluminescence using West Femto maximum detection substrate (Thermo Fisher Scientific).
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Maximum detection rates for Red Delicious apples required use of a Prewitt edge-detection filter.
The detection substrate was nitrophenylphosphatate (Sigma).
Comparison of maximum detection ranges suggests that detection probabilities of small insects are indeed rather similar across bats.
Receivers had a maximum detection range of approximately 800 m [26].
To be able to observe C/EBPα expression even after treatment with high EPO doses, the maximum amount of total protein (40 μg) was loaded in each well, coupled with long autoradiography exposure times and use of a highly sensitivity detection substrate (Pierce's SuperSignal West Dura Extended Duration Substrate; Figure 3A).
BioMax MS Films (Kodak) were used for maximum detection.
Chemokines and growth factors levels over the maximum detection limit were recorded as the maximum value and vice versa.
For maxima detection, we imposed a minimum maxima-to-maxima distance criterion of 10 mm.
After washing, proteins were incubated with an electrochemiluminescent detection kit (Supersignal® West Femto Maximum Sensitivity Substrate; Thermo Scientific, Rockford, IL, USA), and detected using Perkin Elmer's Geliance 600 Imaging System and GeneSnap from SynGene software.
An enhanced chemiluminescence detection agent, SuperSignal West Femto Maximum Sensitivity Substrate, was from Thermo Scientific.
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