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Of the three recombinant LTB.epitope fusion proteins evaluated, antisera against the fusion protein comprising the epitope stretch of 143 175 amino acids gave maximum cross reactivity with the parent protein OmpC.
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Maximum cross-reactivity was 1.6% for LTB4.
The LOQ of PGE2 was 39.1 pg/mL and the maximum cross-reactivity was 70% for PGE1.
The LOQ of 8-iso PGF2α was 6.1 pg/mL and the maximum cross-reactivity was 4.6% for PGF1α.
The LOQ of LTB4 was 11.7 pg/mL and the maximum cross-reactivity was 5.5% for 6-trans-12-epi-LTB4 6-trans-12-epi-LTB4 6-trans-12-epi-LTB4 6-trans-12-epi-LTB4 6-trans-12-epi-LTB4
cross reactivity.
The cross reactivity (CR) was calculated using the formula: IC50 (ROS)/Icompetitortitor) × 100, defining IC50 as the concentration of the competitor that causes 50% reduction of the maximum binding of anti-ROS antibody to the coating conjugates (i.e. 50% of the maximum color signal).
All of these monoclonal antibodies showed cross reactivity to dTMP-GH and Peptide-BSA conjugate.
However, the evolutionary relatedness and structural conservation of these targets often lead to unforeseen cross reactivity.
In itself, antibody cross reactivity is not unusual.
There was also no cross reactivity between the secondary antibodies.
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