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C. glutamicum ATCC 21420, which expressed C. efficiens vanillate O-demethylase, converted FA to PCA with a maximum conversion yield of 62.8% (mol/mol).
The maximum conversion yield (72.2%) was obtained at the optimal condition of 65 °C, 250 rpm, 259 min, 100 mg enzyme, and 2 1 M ratio of eugenol/caprylic acid in solvent-free system.
The maximum conversion yield was 92% when 50 g/liter of pure inulin was incubated with the dual enzyme system containing Xanthomonas enzyme to Pseudomonas enzyme in the ratio of 4 1 for 110 h.
Under optimum conditions, a maximum conversion yield as high as 66% at 50 °C in 5 h using 3 mg/mL of RML-CS/CNWs, and a substrate molar ratio (eugenol: benzoic acid) of 3 1.
The maximum conversion yield (90%±3.55) was obtained by using 400 IU of immobilized lipase and a propanol/gallic acid at a molar ratio of 160 at 52 °C.
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The reaction conditions have been optimized and maximum conversion yields were obtained under conditions of mixed electrolysis conducted in the presence of sodium bromide in water and a platinum anode.
The maximum conversion yields of cellulose and xylan after 72 h were 92.3 and 98.9 %, respectively.
The maximum conversion and yield in the reactor were 85%and63%3%, respectively, using a reaction-separation zone of 30% Lmem/Lmax at molar ratio methanol to oil of 9 1.
It should be noted that inhibition primarily affects the hydrolysis rate and not the maximum conversion or yield, given sufficient time.
The maximum glucan conversion yield obtained (45.61 % for EA pretreated NW by adding rCelStrep to the commercial mix) is higher than or comparable to those reported in recent manuscripts adopting hydrolysis conditions similar to those used in this study.
In conclusion, the maximum polysaccharides conversion yield (12.4%%) was obtained for MIX A. The enzymes rCelStrep, rPoAbf or its evolved variant rPoAbf F435Y/Y446F were added alternatively or in combination to 15 mg/g glucan of MIX 1 (60 % Cellic® CTec3 and 40%% Cellic® HTec3 from Novozymes) for the hydrolysis reactions of pretreated NW.
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