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The reported maximum constriction point of the gap junction sits within the TM domain [10].
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For tracking the gradual changes in PLR response following DT injection, pupil area constrictions over 8 days were normalized to maximum constriction taken as 100%.
The second narrowing occurred horizontally, creating the second constriction point (arrowhead n°2) at x = 140 cm.
As the bees approached this first constriction point, the larger optic flow sum increased.
Figure 2A,B shows the pupil light reflex baseline diameter (pre-stimulus), response latency (delay in pupil constriction after light onset), maximum constriction and recovery (re-dilation after light offset) of two participants at three circadian times (488 nm stimulus light).
The minimum section encountered at an angle of 90° narrowed twice along the tunnel, creating two constriction points: The first narrowing occurred in the vertical plane, creating the first constriction point at x = 80 cm (Figure 4A, arrowhead n°1).
It started 30 cm from the entrance and the maximum constriction (15 cm high by 25 cm wide) occurred 80 cm from the entrance.
The maximum constriction amplitude represents the cone contribution alone to the pupil light reflex [11] because ipRGC latency is >1.78 s for both stimulus lights [39].
The larger optic flow sum increased slightly as the tunnel narrowed and tended to reach a similar value to that recorded before the constriction point.
The hypothesis of the Linear Mixed Model was that the dependent variable (baseline pupil diameter, maximum constriction, PIPR) is not significantly different when the factor (time, repeat) was varied.
The distance between OH atoms of Tyr151, corresponding to the maximum constriction of the pore, were calculated between pairs of residues in opposite M3 helices in the connexon and averaged over the three possible opposite pairs.
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