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Consequently, the maximum consensus identity for the query catalytic domain in BLASTp was observed with a protein of unknown function, A7QWR7/GSVIVT00008775001, from grape (identity-47%, similarity-63%).
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Andreotti backed novelties only after he had secured the maximum consensus.
The story should allow maximum consensus with informative decision-making.
To this end, we propose a modified maximum consensus algorithm in Section V.
The consensus sequence for 1801/UPM was blasted in NCBI BLAST and generated 98 blast hits with the query showing maximum hit identity of 98-99%.
The pairwise nucleotide identity across all sequences in each set is roughly normally distributed around mean 52.8% (minimum mean identity 35.3%, maximum mean identity 93.9%, standard deviation 8%).
Similarly the consensus sequence of strain 1802/KB was also blasted against all nucleotide sequences in GenBank, and out of the 99 blast hit there were four subject sequences with maximum hit identity at 100% with the E-value of 0.0.
The consensus sequence obtained from this alignment showed significant homology to gamma-TMT haplotypes 1, 3, 4 and 5 (GenBank accession nos. DQ229828, DQ229830, and DQ229831 and DQ229834; 8e−18), with 89% maximum sequence identity spanning 18% of the fragment coverage.
The consensus sequence for locus c (388 bp long) showed a significant homology to gamma-TMT haplotypes 4 and 5 (GenBank accessions nos. DQ229831 to DQ229834, 4e-14), with 86% maximum sequence identity spanning 19% of the locus c fragment coverage.
For IAST-1, the locus a sequence showed 100% sequence identity to gamma-TMT haplotype 4. The locus b consensus sequence was 785 bp long and showed a significant homology to gamma-TMT haplotypes 2 and 3 (GenBank accessions nos. DQ229829 and DQ229830, 3e−58), with 94% maximum sequence identity spanning 61% of the locus b fragment coverage.
The maximum target identity between possible target pairs was also relatively low (sequence identity: 23%).
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