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At the time of maximum colony formation (8 to 20 days), counts were performed with an automatic image analysis system (OMNICON FAS IV; Bio-Sys GmbH, Karben, Germany).
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NDGA potently inhibited colony formation in response to both 0.5% FBS and 10% FBS, which induced maximum clonogenic growth in SW 850 and C4-I cells.
ABT-737 was tested up to maximum 50 μM concentration (DMSO > 0.1%) but it was only able to reduce cell colony formation by approximately 20%, 15%and20%0% in DAOY, MED1 and UW228-3 respectively.
Another method of colony formation is sociotomy, or social fragmentation.
colony formation units.
(E) Single cell colony formation.
12302_2016_95_MOESM2_ESM.docx Additional file 2. Yeast colony formation.
Colony formation was evaluated by plating cells onto agar.
The colony formation was then examined and counted.
The plates were incubated at 37 °C to observe colony formation.
(C) The colony formation assay was also applied to confirm the ability of miR-638 on colony formation.
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