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Its initial run, after a dramatic false start in 2008, only reached a maximum collision energy of eight trillion electronvolts.
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Based on SRIM calculations, the maximum in deposited collision energy by H+ and He+ at solar wind energies occurs at respective depths of approximately 11 and 23 nm for plagioclase and 8 and 20 nm for orthopyroxene.
Reporter ion intensities were found to reach a maximum at a normalized HCD collision energy between 50% and 100%.
MS1 was performed using a scan range of 295 450 m/ z, collision energy of 35, maximum injection time of 10 ms, and resolution of 6 × 10.
Collision energy was set to 45, maximum inject time was 250 ms and maximum ion count was 1 × 10 counts.
Full scan Orbitrap spectra (AGC 1x10) were collected from 400 1800 m/z at a resolution of 30,000 with a maximum injection time of 10 ms followed by data-dependent HCD MS/MS (7,500 resolution, 35%% collision energy, 0.1 ms activation time, 100 ms maximum injection time, 1x10 AG C of the 10 most abundant ions using an isolation width of 2.0 Th.
Automatic gain control was set to 1,000,000 for FT-MS and 50,000 for FT-MS, full FT-MS maximum inject time was 500 ms and normalized collision energy was set to 35% with an activation time of 10 ms.
Maximum ion injection time was 100 ms, and normalized collision energy was 35%.
The LTQ was used to acquire MS/MS spectra (3 m/z isolation width, 35% collision energy, 5,000 AGC target, 200 ms maximum ion time).
MS scans were acquired at a resolution of 17,500 with AGC of 2 × 10, maximum injection time of 120 ms, and stepped normalized collision energy (NCE) 50 ± 30%%.
MS data were acquired in negative ionization mode, and the spectra were recorded in full scan (m/z 250 1500) and tandem mass (collision energy of 40% of the instrument maximum) scanning modes.
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