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The disadvantages of apomorphy-based definitions have been extensively discussed (e.g. [ 35, 44]) and recently De Queiroz [ 45] stressed the feasibility of using a stem-based (branch-based, maximum clade) definition to define the name of a total clade.
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The maximum clade credibility chronogram and the summary of the divergence time estimates and clade supports (PP) generated by the BEAST software are shown in Figure 2 and Table 2 Figure 2 Maximum clade credibility chronogram estimated by BEAST.
The first 25% of sampled trees were discarded as burn-in, and the remainder summarised as a maximum clade credibility tree visualised using FigTree v.1.4.0 (Rambaut 2009).
Maximum clade credibility trees were produced using TreeAnnotator v1.5.3.
A maximum clade credibility tree was created with treeannotator and viewed in FigTree.
A final maximum clade credibility tree was determined for each demographic model using TreeAnnotator v1.5.3.
We then adjusted the maximum clade credibility tree to match these expected subgroupings.
Split support was calculated using SumTrees [27] and the maximum clade credibility tree (MCCT the post-burn-in tree with the maximum product of the posterior clade probabilities) was estimated using TreeAnnotator v.1.5.3 [28].
We then calculated the quartets distance from the maximum clade credibility tree to each of these random trees.
The maximum clade credibility tree for analyzing the MCMC data set was annotated by TreeAnotator in the BEAST package.
The maximum clade credibility phylogenetic tree was determined in TreeAnnotator v1.5.4 after excluding an initial 10% of the sample.
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